Schedule

The six-day schedule below is generated from each episode’s frontmatter. Activities fall into three types, shown as labeled groups within each day (the room appears in the group heading once it’s set):

June 8: Introduction, Injection, and EOD Analysis

Name Reading Time Hands-on Time
Self-paced — Location: Home
Introduction: Single-Cell Transcriptomics in Weakly Electric Fish 30 min N/A
Lab protocols — Location: Loeb 160
Optional Reading: EOD Recording: A Practical Overview 30 min 15 min
Optional Reading: 11-KT Injection Protocol 15 min 30 min
Bioinformatics — Location: Loeb 156 (Breakroom)
Examining EOD Duration from Raw Recordings N/A 25 min

June 9: Tissue Dissection

Name Reading Time Hands-on Time
Lab protocols — Location: Loeb 160
Optional Reading: Dissection of Electric Organ, Skin, and Brain for snRNA-seq 15 min 60 min

June 10: Intro to Single-Nucleus RNA-seq Analysis

Name Reading Time Hands-on Time
Self-paced — Location: Home
Introduction to Single-Nucleus RNA-seq 30 min N/A

June 11: Isolating Single Nuclei

Name Reading Time Hands-on Time
Lab protocols — Location: Home
Optional Reading: Isolating Nuclei from Mormyrid Tissues: Theoretical Overview N/A 25 min

June 12: Preprocessing and Batch Correction

Name Reading Time Hands-on Time
Self-paced — Location: Home
snRNA-seq Computation & QC 30 min N/A
Bioinformatics — Location: Loeb 156 (Breakroom)
Preprocessing the Electric Organ snRNA-seq Data N/A 90 min

June 13: Cell-Type Annotation and Differential Expression

Name Reading Time Hands-on Time
Bioinformatics — Location: Loeb 156 (Breakroom)
Cell-Type Annotation and Differential Expression in the Electric Organ N/A 90 min

See Setup for getting your R environment set up!